General Cell Based Assays
Eurofins Discoverx PathHunter eXpress F2RL1 (PAR2; GPR11) HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0235E1CP0M)
The PathHunter F2RL1 (PAR2; GPR11) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. proteases, SLIGRL) based activation of F2RL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged F2RL1 and EA-tagged β-Arrestin-2. Activation of F2RL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MCHR1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0940E3CP5M)
The PathHunter MCHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. MCH) based activation of MCHR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MCHR1 and EA-tagged β-Arrestin-2. Activation of MCHR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric)
Aldo-Keto Reductase (AKR) Activity Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter Human FGFR4 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human FGFR4 Stable Cell Line Functional Kinase Assay (U2OS) measures FGFR4 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Electron Microscopy Sciences Flim Sleeve 135 mm/36 exp with Frosted Open Flap
Polyguard Polypropylene film sleeves are the finest archival quality material, and are guaranteed to give you ultimate protection for your most important film negatives. Polyguard is 100% acid free, as well as being free from optical distortion. It is resistant to water, grease, oil, alkalies, and organic solvents. Polyguard is durable, yet pliable and gentle to the film and will not become brittle, crack, or abrade the film.
Type: Frosted Open Flap
Eurofins Discoverx PathHunter eXpress CXCR2 Activated GPCR Internalization Assay, 2pl Kit. (93-0934E3CP5M)
The PathHunter CXCR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR2. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress A549 G9a Methyltransferase Assay, 10-plate Kit. (96-0003E15CP7L)
The InCELL Hunter eXpress A549 G9a Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of G9a via detection of G9a levels. This product uses EFC technology. The cell line included overexpresses G9a target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMU1R U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0232E3CP0M)
The PathHunter nMU1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuromedin U) based activation of nMU1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged nMU1R and EA-tagged β-Arrestin-2. Activation of nMU1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress ADRA2A CHO-K1 GPCR Assay, 10-Plate Kit. (95-0126E2CP2L)
The cAMP Hunter ADRA2A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2A GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2A, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2A, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NFAT Activation Inhibitor III, Purity: ≥98%, CAS: 3519-82-2, 5 mg
A cell-permeant inhibitor of NFAT signaling; binds calcineurin (Kd = 0.8 µM), preventing it from dephosphorylating NFAT
Meso Scale Discovery R-PLEX AB42 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta40 6E10 Assay is a multiplex immunoassay kit for detecting amyloid beta 40 (Abeta40) and amyloid beta bound by antibody 6E10 in human samples like serum plasma and CSF It uses electrochemiluminescence (ECL) technology for high sensitivity and a broad dynamic range The assay requires 50 L or less of sample and can be completed in 3 5 hours It includes a 96-well plate with capture antibodies detection antibodies standards calibrators controls diluent read buffer wash buffer and plate seals
Eurofins Discoverx PathHunter eXpress GPR97 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0410E2ACP1M)
The PathHunter GPR97 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR97 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR97 and EA-tagged β-Arrestin-2. Activation of GPR97 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 27 Reagent, 500. (93-0563R27B)
AssayComplete Cell Plating 27 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress BRS3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0945E2CP2M)
The PathHunter BRS3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. bombesin) based activation of BRS3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BRS3 and EA-tagged β-Arrestin-2. Activation of BRS3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam ZLN005, PGC-1alpha transcriptional activator, 10MG
MW 250.34 Da, Purity >95%. PGC-1α transcriptional activator. Activates AMPK. Stimulates PGC-1α expression. Increases fat oxidation. Improves glucose and pyruvate tolerance. Increases insulin sensitivity. Shows antidiabetic effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement