General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CDC25A Degradation Assay, 2-Plate Kit. (93-0765E3CP0M)
The PathHunter eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cell line included overexpresses CDC25a target protein fused to ProLabel. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress DARC CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0548E2CP1L)
The PathHunter DARC eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of DARC GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DARC and EA-tagged β-Arrestin-2. Activation of DARC stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPSR1b U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0822E3CP0M)
The PathHunter NPSR1b eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide S) based activation of NPSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPSR1b and EA-tagged β-Arrestin-2. Activation of NPSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical DUB ActIvIty asy KIt 96Wel
Cayman’s DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in a 96-well plate format for multi-sample analysis. EAch kit contains sufficient material for one full 96-well plate assay set-up to be run. Ubiquitin-AMC is not a suitable substrate for all DUBs. Compatibility must be determined by the end user.
Cayman Chemical Glycogen FluorometrIc Detecto
A GnRH receptor antagonist (Kis 6 2.2 and 3.8 nM for the human mouse and rat receptors respectively) selective for the GnRH receptor over a panel of 43 receptors ion channels and enzymes at 1 UM prevents GnRH-induced increases in inositol phosphate production in HEK293 cells expressing the human GnRH receptor (KB 25 nM) oral or intravenous administration decreases plasma LH levels in castrated rats inhibits GnRH-A-induced increases in plasma LH and testosterone levels in non-castrated rats at 20 mg/kg
Eurofins Discoverx PathHunter eXpress rPROKR2 CHO-K1 β-Arrestin GPCR Assay, 10pl Kit. (93-0482E2RCP0L)
The PathHunter rPROKR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prokineticin) based activation of rPROKR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rPROKR2 and EA-tagged β-Arrestin-2. Activation of rPROKR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCRL2 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0333E2ACP1M)
The PathHunter CCRL2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCRL2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCRL2 and EA-tagged β-Arrestin-2. Activation of CCRL2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR120S (Short Isoform) CHO-K1 β-Arrestin GPCR Assay, 2-plate Kit. (93-0924E2CP0M)
The PathHunter GPR120S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. free fatty acids) based activation of GPR120S GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR120S and EA-tagged β-Arrestin-2. Activation of GPR120S stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OXER1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0089E2CP2M)
The cAMP Hunter OXER1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 5-oxo-ETE) based activation of OXER1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OXER1, a naturally Gi/Go coupled GPCR. Upon activation of OXER1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Fumitremorgin C (Tryptoquivaline), Chemosensitizing agent, 250UG
MW 379.5 Da, Purity >98%. Chemosensitizing agent. Potent and selective BCRP inhibitor. Selective AKT inhibitor. Selectively reverses resistance to and potentiates mitoxantrone (ab145668), doxorubicin (ab120629) and topotecan (ab120388) in BCRP-transfected cells. Mycotoxin. Shows antiproliferative effects. Shows tremorgenic effects in vivo. .
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter IL7R/2RG Bioassay Kit, 2-Plate. (93-0997Y13-00081)
The PathHunter IL7R/IL2RG Dimerization Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. IL-7) induced dimerization of IL7R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL7R/IL2RG, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx AssayComplete Thawing Reagent T10, 100. (92-4110TR)
AssayComplete Thawing Reagent T10 is an optimized thawing reagent for revival and recovery of Eurofins DiscoverX cell lines. This reagent ensures optimal post-thaw viability, recovery and assay performance upon thawing. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended thawing reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Cayman Chemical pn-TrkC-Term RabbIt Monc 100uL
A positive allosteric modulator of M1 mAChRs increases ACh-induced calcium flux in CHO cells expressing human M1 receptors (EC50 21 nM) reverses scopolamine-induced cognitive deficits in an object retrieval detour task in rhesus macaques at 0 3 and 1 mg/kg
Meso Scale Discovery R-PLEX Human BMP-6 ASY
The R-PLEX Human BMP-6 Assay Kit from Meso Scale Discovery is an electrochemiluminescent immunoassay for the quantitative measurement of human Bone Morphogenetic Protein 6 (BMP-6) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SECTOR Imager 6000 for high sensitivity detection The kit includes a 96-well plate coated with capture antibody detection antibody calibrator and sample diluent The assay has a range from 1 5 pg/mL to 1000 pg/mL and takes approximately 3 5 hours to complete This product can be used for biomarker research related to bone formation fracture healing and developmental disorders
Eurofins Discoverx PathHunter eXpress GPR85 U2OS β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0409E3CP1M)
The PathHunter GPR85 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR85 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR85 and EA-tagged β-Arrestin-2. Activation of GPR85 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.